Improved PCR amplification/Hhal restriction for unambiguous determination of apolipoprotein E alleles.
نویسندگان
چکیده
We present a modification to the polymerase chain reaction amplification/Hhal restriction isotyping method for human apolipoprotein (apo) E. This method includes a mutagenic forward primer and 5'-end labeling of both primers. These modifications of the original method described by Hixon and Vernier (J Lipid Res 1990;31:545-8) allow sensitive and unambiguous determination of apoE genotypes.
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 41 2 شماره
صفحات -
تاریخ انتشار 1995